DNASE1 Rabbit Polyclonal Antibody

CAT#: R1076PS

DNASE1 rabbit polyclonal antibody, Ig Fraction

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of Deoxyribonuclease 1 from bovine pancreas in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG (goat) R1364HRP and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/1,500 of the reconstitution concentration is suggested.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Deoxyribonuclease 1 from bovine pancreas
• Specificity: This antibody detects bovine Deoxyribonuclease 1. Cross reactivity against Deoxyribonuclease 1 from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresisgive a single precipitin arc against anti-rabbit serum as well as purified and partially purified Deoxyribonuclease 1 from bovine pancreas.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Ig Fraction
  State: Lyophilized IgG fraction
  Preservative: 0.01% (w/V) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 282217 Bovine
  UniProt ID P00639
• Background: Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized.
  DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
• Synonyms: DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I