DNASE1 Rabbit Polyclonal Antibody

CAT#: R1076

DNASE1 rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methods requiring high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Deoxyribonuclease I [Bovine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] code R1364HRP and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1:100 to 1:1,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Deoxyribonuclease I from bovine pancreas.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Deoxyribonuclease I [Bovine Pancreas].
  Cross reactivity against Deoxyribonuclease I from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 0.01% (w/v) Sodium Azide as preservative.
  State: Serum
  State: Lyophilized Serum
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 282217 Bovine
  UniProt ID P00639
• Background: Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized.
  
  DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
• Synonyms: DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I