ansB Rabbit Polyclonal Antibody

CAT#: R1059PS

ansB rabbit polyclonal antibody, Purified

Product Images

Western blot of rabbit anti-L-asparaginase antibody. Lane 1: L-asparaginase. Lane 2: none. Load: 100 ng per lane. Primary antibody: L-asparaginase antibody at 1/1,000 for overnight at 4°C. Secondary antibody: DyLight?TM 649 rabbit secondary antibody at 1/20,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/observed size: 32 kDa for L-asparaginase. Other band (s): L-asparaginase splice variants and isoforms.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution:

  Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/5,000-1/25,000.
  Western blot: 1/500-1/3,000.
  Immunoprecipitation: 1/100.
  This product has been assayed against 1.0 µg of asparaginase [E. coli] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/12,000 of the reconstitution concentration is suggested.

• Reactivities: Escherichia coli
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Asparaginase from E. coli
• Specificity: This antibody detects asparaginase [E. coli]. Cross reactivity against asparaginase from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified asparaginase [E. coli].
• Formulation: 0.01 M Potassium phosphate, 0.14 M Sodium chloride, pH 7.4
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: None
  Preservative: 0.01% Sodium azide
• Reconstitution Method: Restore with 0,1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00805
• Synonyms: ansA, L-asparaginase I, L-ASNase I