ADH1 Rabbit Polyclonal Antibody

CAT#: R1049BS

ADH1 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  Antibody has been assayed against 1.0 ug of Alcohol dehydrogenase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
• Reactivities: Yeast
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Alcohol dehydrogenase from yeast
• Specificity: This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined.
  Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Lyophilized purified Ig faction
  Stabilizer: 10 mg/m BSA (immunoglobulin and protease free)
  Preservative: 0.01% Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00330
• Synonyms: Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947