ALB Rabbit Polyclonal Antibody

CAT#: R1048PS

ALB rabbit polyclonal antibody, Ig Fraction

Product Images

Western blot of Rabbit Anti-Bovine Serum Albumin secondary antibody. Lane 1: BSA. Lane 2: None. Load: 50 ng per lane. Primary antibody: BSA antibody at 1/1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: 5% Goat Serum for 30 min at RT. Predicted/Observed size: 66 kDa, 61 kDa for BSA. Other band (s): None

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/20,000-1/100,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Reactivities: Bovine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Native Protein Bovine Serum Albumin.
  Bovine Serum Albumin antibody was produced by repeated immunizations with bovine serum albumin.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum).
  Cross reactivity against Albumin from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  State: Ig Fraction
  State: Lyophilized purified IgG fraction from monospecific antiserum
  Stabilizer: None
  Preservative: 0.01% (w/v) Sodium Azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multistep Process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated before
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Dilute only prior to immediate use.
  Centrifuge product if not completely clear after standing at room temperature.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 280717 Bovine
  UniProt ID P02769
• Background: Bovine Serum Albumin antibody detects BSA. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca²⁺, Na⁺, K⁺, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma; it typically binds about 80% of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
• Synonyms: ALB, BSA, HSA, Serum Albumin