Lyve1 Rabbit Polyclonal Antibody

CAT#: DP3513

Lyve1 rabbit polyclonal antibody, Purified

Product Images

Figure 5. Western Analysis of anti-Mouse LYVE-1 Antibody. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.

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  Figure 2. Immunohistochemistry with Cryo sections from Rat Skin using anti-Mouse LYVE-1 antibody

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  Figure 3. Immunohistochemistry with Cryo sections from Rat Tumor Tissue using anti-Mouse LYVE-1 antibody

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  Figure 1. Staining of mouse colon using a CD31 antibody (green) and Lyve-1 antibody (red). Picturted originate from Dr. Ulrike Fiedler and Stefanie Koidel, Dept. of Vascular Biology and Angiogenesis Research Tumor Biology center, Freiburg, Germany.

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  Figure 4. LYVE-1 and CD31 staining on frozen sections (5um) of the Mouse prostate. The experiments were performed by Scott Gerber & Edith Lord, PhD, University of Rochester, USA.

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Specifications

Product Data

• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA (1-15 µg/ml).
  Western blot (1-5 µg/ml).
  FACS analysis (3-10 µg/ml).
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections (1-5 µg/ml).
  For formalin-fixed, paraffin-embedded sections use the immunogen affinity purified antibody Cat.-No DP3513P.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Mouse soluble LYVE-1 (Ala24-Gly228) produced in insect cells (Cat.-No DA3524).
  This recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
• Specificity: This antibody detetcs Mouse and Rat Lyve-1.
  This antibody is not reactive with Human LYVE-1.
• Formulation: PBS, pH 7.2 without preservatives or stabilizers
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein G Chromatography (+his tag depletetion)
• Conjugation: Unconjugated
• Storage: The lyophilized IgG is stable at 2-8°C for one month and at -20°C for longer.
  When reconstituted the antibody is stable for at least six weeks at 2-8°C.
  For longer store in aliquots at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 114332 Mouse
  UniProt ID Q8BHC0
• Background: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212- esidue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE- molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymphspecific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
• Synonyms: LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1