Vegfc Rabbit Polyclonal Antibody

CAT#: DP3507

Vegfc rabbit polyclonal antibody, Azide Free

Product Images

VEGF-C Sandwich-ELISA using the polyclonal anti-rat VEGF-C antibody as capture antibody and recombinant Rat VEGF-C ( DA3519X) as standard. The Biotinylated rabbit anti-rat VEGF-C (No B) was used as detection antibody.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: 2.5 µg/ml, allows the detection of 0.5-1.0 ng/well of recombinant rat VEGF-C.
  Western Blot: 2-5 μg/ml.
  
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Rat VEGF-C native protein.(Asp101-Ile221) derived from insect cells (Cat.-No DA3519X)
• Specificity: The antibody will react with Rat VEGF-C and will cross-react with Human and Mouse VEGF-C proteins.
• Formulation: PBS, pH 7.4, containing no preservative.
  State: Azide Free
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of > 0.5 mg/ml.
• Purification: Protein A affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for 2 weeks
  or (in aliquots) at -20°C up to 6 month.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: vascular endothelial growth factor C
• Database Link:
  Entrez Gene 114111 Rat
  UniProt ID O35757
• Background: VEGF-C, a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. It forms cell surfaced-associated non-covalent disulfide linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. Recombinant, fully processed rat VEGF-C is a 16-18 kDa non-disulfide linked homodimeric protein consisting of two 116 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition.
• Synonyms: VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, Flt4 ligand