LYVE1 Rabbit Polyclonal Antibody
LYVE1 rabbit polyclonal antibody, Aff - Purified
|Applications||ELISA, FC, IF, IHC, WB|
|Recommended Dilution||Immunohistochemistry on Frozen and Paraffin Embedded Sections (1-5 µg/ml).
Heat mediated antigen retrieval is recommended when staining paraffin embedded sections.
Western blot (1-2 µg/ml).
ELISA (1-15 µg/ml).
Flow Cytometry (3-20 µg/ml).
|Immunogen||Highly pure recombinant Human soluble LYVE-1 produced in insect cells (Cat.-No DA3525).
It consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).
|Specificity||This antibody will detect LYVE-1 on the surface of lymphatic endothelial cells by Immunohistochemistry.
It detects a 70 kD LYVE-1 band in Western blotting.
This antibody is not reactive with Mouse LYVE-1.
|Formulation||5mM PBS, pH 7.2 without preservatives or stabilizers
State: Aff - Purified
State: Lyophilized purified IgG fraction
|Reconstitution Method||Restore in sterile water to a concentration of 0.1-1.0 mg/ml|
|Purification||Antigen Affinity Chromatography|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels.|
|Synonyms||LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.