LYVE1 Rabbit Polyclonal Antibody

SKU DP3500PS

LYVE1 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen and Paraffin Embedded Sections (1-5 µg/ml).; Heat mediated antigen retrieval is recommended when staining paraffin embedded sections.; Western blot (1-2 µg/ml).; ELISA (1-15 µg/ml).; Flow Cytometry (3-20 µg/ml).
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Human soluble LYVE-1 produced in insect cells (Cat.-No DA3525).; It consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).
• Specificity: This antibody will detect LYVE-1 on the surface of lymphatic endothelial cells by Immunohistochemistry.; It detects a 70 kD LYVE-1 band in Western blotting.; This antibody is not reactive with Mouse LYVE-1.
• Buffer: 5mM PBS, pH 7.2 without preservatives or stabilizers; State: Aff - Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Antigen Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: lymphatic vessel endothelial hyaluronan receptor 1
• Database Link:
  Q9Y5Y7
• Background: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels.
• Synonyms: LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1

Reference Data