HOXC4 Mouse Monoclonal Antibody [Clone ID: OTI2D5]

CAT#: CF809708

Carrier-free (BSA/glycerol-free) HOXC4 mouse monoclonal antibody,clone OTI2D5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HOXC4 (Cat# RC223682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC4 (Cat# TA809708)(1:2000). Positive lysates LY406994 (100ug) and LC406994 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-HOXC4 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809708) (1:2000)

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Specifications

Product Data

• Clone Name: OTI2D5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HOXC4 (NP_705897) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: homeobox C4
• Database Link:
  NP_705897
  Entrez Gene 15423 MouseEntrez Gene 3221 Human
  UniProt ID P09017
• Background: This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene, HOXC4, is one of several homeobox HOXC genes located in a cluster on chromosome 12. Three genes, HOXC5, HOXC4 and HOXC6, share a 5' non-coding exon. Transcripts may include the shared exon spliced to the gene-specific exons, or they may include only the gene-specific exons. Two alternatively spliced variants that encode the same protein have been described for HOXC4. Transcript variant one includes the shared exon, and transcript variant two includes only gene-specific exons. [provided by RefSeq, Jul 2008]
• Synonyms: cp19; HOX3; HOX3E

Reference Data

• Protein Families: Transcription Factors