HLCS Mouse Monoclonal Antibody [Clone ID: OTI1E4]

SKU CF504422

Carrier-free (BSA/glycerol-free) HLCS mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)

Product Data

• Clone Name: OTI1E4
• Application: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 80.6 kDa
• Gene Name: holocarboxylase synthetase
• Database Link:
  NP_000402
  P50747
• Background: This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Synonyms: HCS

Reference Data

• Protein Pathways: Biotin metabolism, Metabolic pathways