CAMLG Mouse Monoclonal Antibody [Clone ID: OTI1A3]

CAT#: CF504329

Carrier-free (BSA/glycerol-free) CAMLG mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CAMLG (Cat# RC218292, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMLG (Cat# TA504329). Positive lysates LY419768 (100ug) and LC419768 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CAMLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504329)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CAMLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504329)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CAMLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504329)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CAMLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504329)

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  Anti-CAMLG mouse monoclonal antibody (TA504329) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CAMLG (RC218292).

• HEK293T cells transfected with either RC218292 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CAMLG antibody (TA504329), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CAMLG(NP_001736) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.8 kDa
• Gene Name: calcium modulating ligand
• Database Link:
  NP_001736
  Entrez Gene 819 Human
  UniProt ID P49069
• Background: The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. [provided by RefSeq]
• Synonyms: CAML

Reference Data

• Protein Families: Druggable Genome