EIF4EL3 (EIF4E2) Mouse Monoclonal Antibody [Clone ID: OTI4E7]

CAT#: CF502431

Carrier-free (BSA/glycerol-free) EIF4E2 mouse monoclonal antibody, clone OTI4E7 (formerly 4E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E2 (RC201391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E2. Positive lysates LY401518 (100ug) and LC401518 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-EIF4E2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502431)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502431)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502431)

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Specifications

Product Data

• Clone Name: OTI4E7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF4E2(NP_004837) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.2 kDa
• Gene Name: eukaryotic translation initiation factor 4E family member 2
• Database Link:
  NP_004837
  Entrez Gene 26987 MouseEntrez Gene 9470 Human
  UniProt ID O60573
• Synonyms: 4E-LP; 4EHP; EIF4EL3; IF4e

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway