OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI1B12]

  • Product Brand Image
Be the first to review this product
SKU
CF500021
Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
Bulk/Customize

View other "Cytokeratin 8 or NM_002273" antibodies

Specifications
Specifications
Product Data
Clone Name OTI1B12
Application IF, IHC, Simple Western, WB
Recommended Dilution WB 1:2500~5000, IHC 1:50 - 1:150, IF 1:50 - 1:100, Simple Western 1:20-1:50
Simple Western 1:50-1:100
Reactivity Dog, Human, Monkey, Mouse, Rat
Antibody Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 53.5 kDa
Gene Name keratin 8
Database Link
Background Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Synonyms CARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Reference Data
Protein Categories ADC Targets, Intracellular Proteins
Protein Families Druggable Genome
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "OTI1B12" antibodies (4)
SKU Description Size Price
TA500021 CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12) 100 ul
$447.00
TA500021AM CK8 mouse monoclonal antibody, clone 1B12, Biotinylated 100 ul
$509.00
TA500021BM CK8 mouse monoclonal antibody, clone 1B12, HRP conjugated 100 ul
$509.00
TA500021S CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12) 30 ul
$200.00

file-alt Citations

The Prognostic Value of Cytokeratin and Sal-Like Protein 4 Expression in Hepatocellular Carcinoma and Intra-Hepatic Cholangiocarcinoma in Taiwan
Lai, Cheng, Lee et al
Int J Med Sci (2018) 15 (14), 1746-1756 DOI: 10.7150/ijms.28440
Cited by 10
AI Summary: In this study, OriGene products—including anti-cytokeratin 7, anti-cytokeratin 8, anti-cytokeratin 18, anti-cytokeratin 19, and anti-SALL4 antibodies—were used for immunohistochemical detection of protein expression in liver cancer tissues, enabling the assessment of their prognostic value in hepatocellular carcinoma and intra-hepatic cholangiocarcinoma.
FigureFigureFigure
Stress chaperone mortalin contributes to epithelial-mesenchymal transition and cancer metastasis
Na, Kaul, Ryu et al
Cancer Res. (2016)
AI Summary: OriGene’s Keratin 8 antibody was used in this study to assess changes in epithelial marker expression, helping to demonstrate mortalin’s role in promoting epithelial-mesenchymal transition and cancer cell invasiveness.
Snippet: Cambridge, MA);hnRNP-K, MMP-2, MMP-3 &fibronectin (Santa Cruz Biotechnology, Santa Cruz, CA);Keratin 8 (OriGene Technologies, Rockville, MD);Keratin 14 (Anaspec, San Jose, CA);Keratin 18, myc-tag and β-actin (Cell Signaling Technology), and Alexa Fluor ... Cite SaveSaving
The Prognostic Value of Cytokeratin and Sal-Like Protein 4 Expression in Hepatocellular Carcinoma and Intra-Hepatic Cholangiocarcinoma in Taiwan
Lai, Cheng, Lee et al
Int J Med Sci (2018)
Snippet: … CK8, Mouse monoclonal, TA500021, IgG2b, OriGene, 1:200, ER2 20 min. CK18, Mouse monoclonal, TA500015, IgG1, OriGene, 1:200, ER2 20 min. SALL4, Rabbit monoclonal, EP299, IgG, ZETA, 1;CK19 Mouse monoclonal BA17 IgG1 ZETA 1:100 ER2 20 min CK8 Mouse monoclonal TA500021 IgG2b OriGene 1:200 ER2 20 min CK18 Mouse monoclonal TA500015 IgG1 OriGene 1:200 ER2 20 min SALL4 Rabbit monoclonal EP299 IgG ZETA 1:200 ER2 20 min View as HTML
IHC
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products