Fn1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, R |
|---|---|
| Recommended Dilution | This antibody can be used on Frozen and Paraffin Embedded Tissues: IFA (1/80), IHC(P) (1/500) After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation time: IHC(P) 60 min at RT or 2-8°C over night. Also suitable for ELISA (1/200) and RIA. |
| Reactivity | Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Purified fibronectin from Murine plasma. |
| Specificity | Murine Fibronectin 100% Murine Collagen type I, III and IV all < 0.1% Murine Laminin < 0.1% (RIA at 1/10000 dilution). |
| Buffer | PBS without preservative or stabilizer. State: Purified State: Lyophilized purified Ig fraction. |
| Reconstitution Method | Restore with 0.5 ml distilled water, dilute further with appropriate antibody diluent. |
| Concentration | ~1.0 mg/ml (after reconstitution) |
| Purification | Affinity Chromatography. |
| Conjugation | Unconjugated |
| Storage | Sore the antibody undiluted 2-8°C for one month or (in aliquots) at -20°C longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Ambient |
| Gene Name | fibronectin 1 |
| Database Link | |
| Background | Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. |
| Synonyms | FN1, Cold-insoluble globulin, CIG |
| Reference Data | |
| Protein Categories | ADC Targets, Growth Factors, Intracellular Proteins, Secreated Proteins |
Reviews
Documents
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| FAQs |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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