Fibronectin Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IF, IHC, R |
|---|---|
| Recommended Dilution | IF: 1:40. IHC: 1:200 - 1:600 for paraffin sections, 60 min at RT or 2-8°C over night. ELISA. RIA. Positive control: Chicken skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. |
| Reactivity | Chicken |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Purified fibronectin from chicken plasma |
| Specificity | Chicken fibronectin 100%, chicken collagen type I, III and V < 0.1% (RIA at 1:10000 dilution). This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. |
| Buffer | State: Purified State: Lyophilized Ig fraction |
| Reconstitution Method | Reconstitute with 0.5 ml distilled water, for further dilution use appropriate antibody diluent. |
| Concentration | lot specific |
| Purification | Ion exchange chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Background | Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. |
| Synonyms | FN1, Cold-insoluble globulin, CIG |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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