COL1A1 Rabbit Polyclonal Antibody

CAT#: BP8000S

COL1A1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA.
  ELISA: 1/400 (OD ≥ 500).
  Immunoblotting (Western blot).
  Immunofluorescence (1/80).
  Immunohistochemistry on Paraffin embedded tissues: 1/100-1/500.
  Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Incubation Time: 60 min at RT or 2-8°C overnight.
  Positive Control: Bovine skin.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type I from Bovine skin.
• Specificity: Bovine Collagen type I: 100%.
  Bovine Collagen II, IX, XI: < 0.1%
  Sheep Collagen type I: 50% (IHC estimated)
  Bovine Fibronectin: < 0.1%
  Human Collagen type I: < 2%
  Chicken Collagen type I: < 0.1%
  (Estimates except Sheep Collagen I from solid phase RIA at dilution 1/500).
  
  Even though cross-reactivity with Human is low in RIA, the antibody has to be expected to be cross-reactivive to Human Collagen type I as well as to Pig Collagen type I in IHC.
  
  
• Formulation: PBS without BSA and preservatives
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore with 0.1 ml (BP8000S) or 0.5 ml (BP8000) distilled water.
  For further dilution use appropriate antibody diluent.
• Concentration: ~1.0 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 282187 Bovine
  UniProt ID P02453
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type I Collagen (95 kDa) usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen