VC1456 Rabbit Polyclonal Antibody

CAT#: BP2193

VC1456 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IP
• Recommended Dilution: ELISA: Titer: >1:1,000.
  Also suitable for conjugation purposes.
  Immunoprecipitates toxin in gel techniques.
• Reactivities: Vibrio cholerae
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified choleragenoid.
• Specificity: Beta subunit of Cholera toxin.
• Formulation: 0.01M PBS, pH 7.2 with 0.09% sodium azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction (>95% pure) .
• Concentration: lot specific
• Purification: Protein A chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P01556
• Background: The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge.
  The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
• Synonyms: Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, ctxB, toxB, VC_1456