Dopamine D2 Receptor (DRD2) (Short Isoform, 239-246) Rabbit Polyclonal Antibody

CAT#: BP122S

Dopamine D2 Receptor (DRD2) (Short Isoform, 239-246) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Immunohistochemistry: Suitable for immunocytochemical detection of the D2s dopamine receptor : this antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at 1:500 dilution. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.
  Western Blot of the D2s dopamine receptor.
  Western Immunoblots using whole rat brain homogenate resulted in a single band being detected at ~48 kD at 1:800 dilution.
  Immunocytochemisty.
  ELISA.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: D2s (Ac239-Cys²⁴⁷) covalently attached to a carrier protein
• Specificity: Specific for Dopamine D2s Receptor (a.a. 239-246). This antisera has been characterized by immunocytochemical, Western immunoblot and ELISA techniques.
  Cross-reactivity:
  D2s Dopamine Receptor (239-246): 100%
  D2s Dopamine Receptor: 50%
  D2L Dopamine Recetpor: 0%
  D1 Dopamine Receptor: (9-21) 0%
  D1 Dopamine Receptor: 0%
  D3 Dopamine Receptor: (2-10) 0%
  D3 Dopamine Receptor: 0%
  D4 Dopamine Receptor (176-185): 0%
  D4 Dopamine Receptor: 0%
  D5 Dopamine Receptor (23-35): 0%
  D5 Dopamine Receptor: 0%
• Formulation: State: Serum
  State: Lyophilized neat serum
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer for more dilute antisera.
• Conjugation: Unconjugated
• Storage: Lyophilized: Store at 2-8°C (preferably in a dessicator).
  Reconstituted: Aliquot and store at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: dopamine receptor D2
• Database Link:
  Entrez Gene 1813 Human
  UniProt ID P14416
• Synonyms: DRD2

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Gap junction, Neuroactive ligand-receptor interaction