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Adgre1 Rat Monoclonal Antibody [Clone ID: BM8]

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SKU
BM4007B
Adgre1 rat monoclonal antibody, clone BM8, Biotin
Size:
100 ug
Conjugation:
Biotin Unconjugated FITC
  $880.00
2 Weeks*
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View other "Adgre1 or NP_034260" antibodies

Specifications
Specifications
Product Data
Clone Name BM8
Application FC, IF, IHC
Recommended Dilution Immunohistochemistry on Frozen Sections: 1.0 µg/ml (1/200).
Immunohistochemistry on Paraffin Sections: not tested, but work well with the purified antibody using Proteinase K pretreatment.
Recommended Positive Control: Mouse spleen.
Has been described to work in FACS.
Reactivity Mouse
Antibody Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Cultured Macrophages.
Specificity This antibody is useful for the detection of major subpopulations of resident tissue macrophages. It is also suitable for the detection of blood monocytes and other monocytic cells isolated from different sources by FACS.
This clone is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model.
Antigen Distribution:
Isolated Cells: expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.
Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
Buffer PBS, pH 7.2
Label: Biotin
State: Lyophilized purified IgG fraction
Stabilizer: 5 mg/ml BSA
Preservative: None
Reconstitution Method Restore with 0.5 ml distilled water
Concentration 0.2 mg/ml (after reconstitution)
Purification Affinity Chromatography
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Gene Name adhesion G protein-coupled receptor E1
Database Link
Background F4/80 protein binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. F4/80 is not expressed by lymphocytes or polymorphonuclear cells.
Synonyms Emr1, Gpf480
Reference Data
Protein Categories GPCR, Intracellular Proteins, Membrane Proteins
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Documents
Resources

file-alt Citations

Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody
Stangl, Gehrmann, Riegger et al
Proc Natl Acad Sci U S A (2011) 108 (2), 733-8 DOI: 10.1073/pnas.1016065108
Cited by 205
AI Summary: In this study, OriGene's recombinant mouse Hsp70 protein (Cat# TP301482) was used to confirm the specificity of the cmHsp70.1 antibody for its target epitope on tumor cells.
Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization
Torzewski, Shaw, Han et al
Arterioscler Thromb Vasc Biol (2004) 24 (12), 2307-12 DOI: 10.1161/01.ATV.0000149378.98458.fe
AI Summary: In this study, OriGene’s mouse anti-human α-actin monoclonal antibody (clone 1A4) was used for immunostaining to identify smooth muscle cells in atherosclerotic plaques, helping to assess changes in plaque composition associated with stabilization.
Showing 1-2 of 2 papers.
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