WTIP (C-term) Rabbit Polyclonal Antibody

CAT#: AP54573PU-N

WTIP (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

WTIP Antibody (C-term) western blot analysis in NCI-H460, WiDr cell line lysates (35 ug/lane). This demonstrates the WTIP antibody detected the WTIP protein (arrow).

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  WTIP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WTIP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of WTIP Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
  Immunohistochemistry on paraffin sections: 1:10~50.
  Immunofluorescence: 1:10~50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human WTIP
• Specificity: This antibody detects WTIP (C-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Wilms tumor 1 interacting protein
• Database Link:
  Entrez Gene 126374 Human
  UniProt ID A6NIX2
• Background: WTIP may monitor slit diaphragm protein assembly, a specialized adherens junction characteristic of podocytes. In case of podocyte injury, it shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype (By similarity).
• Synonyms: Wilms tumor protein 1-interacting protein
• Note: Molecular Weight: 67454 Da