DARS (N-term) Rabbit Polyclonal Antibody

CAT#: AP51185PU-N

DARS (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of DARS1 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). This demonstrates the DARS antibody detected the DARS protein (arrow).

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  Western blot analysis of DARS (arrow) using DARS1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.

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  Formalin fixed and paraffin embedded human liver tissue stained with DARS1 Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of DARS1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunofluorescence: 1/10-/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of Human DARS.
• Specificity: This antibody recognizes Human DARS (N-term).
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: aspartyl-tRNA synthetase
• Database Link:
  Entrez Gene 1615 Human
  UniProt ID P14868
• Background:
  Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.
• Synonyms: DARS, PIG40, AspRS, Aspartate-tRNA ligase
• Note: Molecular Weight: 57136 Da

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis