CYC1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51160PU-N

CYC1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in mouse brain tissue lysates (35ug/lane) using Cytochrome C1 antibody. (C-term). This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).

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  Western blot analysis in Hela cell line lysates (35ug/lane) using Cytochrome C1 antibody. (C-term). This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).

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  Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) using Cytochrome C1 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution:

  This antibody is suitable for flow cytometry and Western blotting.
  The suggested dilution is:
  Flow cytometry: 1/10-1/50.
  Western blotting: 1/100-1/500.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 270-299aa) of human Cytochrome C1/ CYC1.
• Specificity: This antibody detects Cytochrome C1 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a protein A column; followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: cytochrome c1
• Database Link:
  Entrez Gene 66445 MouseEntrez Gene 1537 Human
  UniProt ID P08574
• Background: This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
• Synonyms: CYC1, Cytochrome b-c1 complex subunit 4, Complex III subunit 4, Complex III subunit IV
• Note: Molecular Weight: 35422 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease