GFP Rabbit Polyclonal Antibody

SKU AP33450PU-N

GFP rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: WB
• Recommended Dilution: Western blot: 1-2 µg/ml.
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant GFP protein.; The anti-GFP antibody was generated by immunization of rabbits with highly purified recombinant GFP protein produced in E. coli.
• Specificity: The antibody was made against GFP from the jellyfish Aequorea Victoria using the whole native protein as an antigen.
• Buffer: PBS, pH 7.4, without preservatives; State: Aff - Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Database Link:
  P42212
• Background: Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
• Synonyms: Green fluorescent protein, GFP-Tag

Reference Data