Pgf Rabbit Polyclonal Antibody

CAT#: AP31725PU-S

Pgf rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemical staining of PlGF in paraffin-embedded mouse placenta embryo (a and b) showing intense cytoplasmic staining in mouse placenta at E9. In the embryo a positive signal was observed in endothelial structures of highly vascularized organs. Cross-reactivity of antibody was disproved as staining of human placenta did not reveal any signal ©. Lower panel shows higher magnification of boxes in a-c. The experiments were performed by Dr. Frank Bicker from the research group ??Molecular Signal Transduction?? (Prof. Dr. Mirko HH Schmidt), Institute of Microscopic Anatomy and Neurobiology, University Medical Center of Johannes Gutenberg University Mainz, Germany.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 2-5 μg/ml.
  No cross-reactivity with recombinant Human PlGF under non-reducing conditions is observed.
  Immunofluorescence/Immunohistochemistry.
  Immunohistochemistry on Paraffin Sections.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (> 95%) recombinant Mouse PlGF (Ala24-Pro158) derived from Sf9 insect cells (Cat.-No AR26011PU-L).
• Specificity: Recognizes Mouse PlGF. Other species not tested.
• Formulation: 5mM PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Antigen Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: placental growth factor
• Database Link:
  Entrez Gene 18654 Mouse
  UniProt ID P49764
• Background: Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF1, PlGF2 and PlGF3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF2 has been identified. Mouse PlGF shares 65% amino acid identity with human PlGF2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
• Synonyms: PGFL, PLGF, PlGF