ERK1 / ERK2 (N-term) Rabbit Polyclonal Antibody
$600.00
2 Weeks*
Specifications
| Product Data | |
| Application | IHC, IP, WB |
|---|---|
| Recommended Dilution | Western blot: At 0.5-1μg/ml with the appropriate system to detect MAPK1/3 in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect MAPK1/3 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide mapping at the N-terminal of the human MAPK1+3 |
| Specificity | This antibody detects ERK1 / ERK2 at N-term. No cross reactivity with other proteins. |
| Buffer | 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction |
| Reconstitution Method | 0.2ml of distilled water will yield a concentration of 500μg/ml. |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
| Storage | Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Background | MAPK1(ERK2) shares high homology with MAPK3(ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases also known as Erks have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2. |
| Synonyms | ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK |
| Reference Data | |
Reviews
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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