Thermonuclease Rabbit Polyclonal Antibody

CAT#: AP21415AF-N

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Thermonuclease rabbit polyclonal antibody, Azide Free



USD 240.00


Availability*
2 Weeks

Size
    • 10 mg


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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Recommended Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
Reactivity Staphylococcus aureus
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Nuclease isolated and purified from Staphylococcus aureus.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity This antibody is a polyclonal immunologic reagent to Nuclease from Staphylococcus aureus.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized purified hyperimmune IgG fraction
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration 10.0 mg/ml
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Native Stapylococcus aureus Nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3'-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems.
Synonyms TNase, Nuclease, nuc
Reference Data

Citations (1)

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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