Thermonuclease Rabbit Polyclonal Antibody

CAT#: AP21415AF-N

Thermonuclease rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
  Recommended Dilutions:
  Non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
• Reactivities: Staphylococcus aureus
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Nuclease isolated and purified from Staphylococcus aureus.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: This antibody is a polyclonal immunologic reagent to Nuclease from Staphylococcus aureus.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized purified hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00644
• Background: Native Stapylococcus aureus Nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3'-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems.
• Synonyms: TNase, Nuclease, nuc