Thermonuclease Rabbit Polyclonal Antibody
Thermonuclease rabbit polyclonal antibody, Azide Free
|Applications||ELISA, ID, IF, IP, R, WB|
|Recommended Dilution||This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
|Immunogen||Nuclease isolated and purified from Staphylococcus aureus.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
|Specificity||This antibody is a polyclonal immunologic reagent to Nuclease from Staphylococcus aureus.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
|Formulation||PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized purified hyperimmune IgG fraction
|Reconstitution Method||Restore by adding 1.0 ml of sterile distilled water|
|Purification||Ammonium Sulphate Precipitation and Ion Exchange Chromatography|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Native Stapylococcus aureus Nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3'-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems.|
|Synonyms||TNase, Nuclease, nuc|
|The use of this Antibodies has been cited in the following citations:|
Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions
,Miettinen TP, Bjvrklund M,
Nucleic Acids Res. 2018
,PubMed ID 25550424