PRSS1 Rabbit Polyclonal Antibody

CAT#: AP21396BT-N

PRSS1 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or histochemical techniques).
  Recommended Working Dilutions: vary widely, but may be between 1/1000 and 1/5000.
• Reactivities: Bovine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Trypsin isolated and purified from Bovine pancreas.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity:
  Biotin conjugated IgG fraction of Polyclonal Rabbit antiserum to Trypsin from Bovine pancreas.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized Hyperimmune purified IgG fraction
  Label: Marker: N-Hydroxysuccinimidobiotin.
  Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps.
  After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life
  Molar radio: Biotin/ IgG ~4.4
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 780933 BovineEntrez Gene 615026 Bovine
  UniProt ID P00760
• Synonyms: TRP1, TRY1, TRYP1, Trypsin I, Cationic trypsinogen, Beta-trypsin, Serine protease 1