Thermolysin Rabbit Polyclonal Antibody

CAT#: AP21392PU-N

Thermolysin rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
  Recommended Working Dilutions: Vary widely, but may be between 1/10,000 and 1/100,000.
• Reactivities: Bacillus sp.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Thermolysin isolated and purified from Bacillus thermoproteolyticus rokko.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Affinity purified antibodies of polyclonal rabbit antiserum to Thermolysin from Bacillus thermoproteolyticus rokko.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
• Formulation: PBS, pH 7.2 stabilized with 0.5 % Dextran without preservatives and foreign proteins
  State: Aff - Purified
  State: Lyophilized purified hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml of sterile distilled water
• Concentration: 2.0 mg/ml (after reconstitution)
• Purification: Solid Phase Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00800
• Synonyms: Thermostable Neutral Proteinase, npr