Phospholipase C / PLC Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Recommended Dilution | Can be used for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques). Working dilutions: vary widely, but may be between 1/1000 - 1/20000. |
| Reactivity | Bacillus sp. |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Phospholipase C isolated and purified from Bacillus cereus. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase C from Bacillus cereus. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. |
| Buffer | PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized purified IgG fraction. Molar radio: Biotin/IgG ~5.4 |
| Reconstitution Method | Restore by adding 1,0 ml of sterile distilled water. |
| Concentration | lot specific |
| Purification | Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and DEAE-Chromatography. |
| Conjugation | Biotin |
| Storage | Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Synonyms | Cereolysin A |
| Reference Data | |
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