Luciferase Rabbit Polyclonal Antibody

SKU AP21341BT-N

Luciferase rabbit polyclonal antibody, Biotin

Product Data

• Application: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/10,000).
• Reactivity: Photinus pyralis
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Luciferase isolated and purified from Photinus pyralis. ; Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.; Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Buffer: PBS, pH 7.2 without preservatives and foreign proteins.; Label: Biotin; State: Lyophilized purified IgG fraction.; Molar radio: Biotin/IgG: ~1.7
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term. ; If a slight precipitation occurs upon storage, this should be removed by centrifugation.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Database Link:
  P08659
• Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Reference Data