3-beta-HSD Rabbit Polyclonal Antibody

CAT#: AP21324BT-N

3-beta-HSD rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
  Recommended Working Dilutions:
  Non-precipitating antibody-binding techniques: at least 1/400.
• Reactivities: P. testosteroni
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: beta-Hydroxysteroid Dehydrogenase isolated and purified from Pseudomonas testosteroni cells.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: beta-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni cells.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~5.2
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P19871
• Synonyms: 3-beta-hydroxysteroid dehydrogenase