DBF4 Rabbit Polyclonal Antibody

SKU AP20463PU-N

DBF4 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.; Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Dbf4 protein.; (region surrounding Ile18)
• Buffer: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 77 kDa
• Gene Name: DBF4 zinc finger
• Database Link:
  Q9UBU7
• Background: The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.
• Synonyms: DBF4A, ZDBF1, Chiffon homolog A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle