Cripto1 (TDGF1) Goat Polyclonal Antibody

CAT#: AP20147PU-N

Cripto1 (TDGF1) goat polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry: CRIPTO Antibody staining of Paraffin Embedded Human Placenta at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit)
  Immunohistochemistry on Paraffin Sections: 3-5 µg/ml.
  In paraffin embedded Human Placenta shows staining assocated with nuclei in some trophoblasts and weak cytoplasm staining in cytotrophoblasts.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003203.1.
• Specificity: Recognizes CRIPTO
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: teratocarcinoma-derived growth factor 1
• Database Link:
  Entrez Gene 6997 Human
  UniProt ID P13385
• Background: Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGFbeta) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.
• Synonyms: Cripto-1 growth factor, CRGF