ADA Rabbit Polyclonal Antibody

CAT#: AP20098AF-N

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ADA rabbit polyclonal antibody, Azide Free

USD 293.00

2 Weeks

    • 10 mg

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Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/10,000).
Reactivities Bovine
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Adenosine Deaminase isolated and purified from Calf Intestine.
Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins.
State: Azide Free
State: Lyophilized IgG fraction.
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water.
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation Unconjugated
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability Shelf life: one year from despatch.
Background ADA (Adenosine deaminase) is an enzyme involved in the purine catabolic pathway. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Defects in ADA are a cause of autosomal recessive severe combined immuno-deficiency (SCID).
Synonyms Adenosine aminohydrolase, ADA
Reference Data
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