ADA Rabbit Polyclonal Antibody
ADA rabbit polyclonal antibody, Azide Free
|Applications||ELISA, ID, IF, IP, R, WB|
|Recommended Dilution||This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/10,000).|
|Immunogen||Adenosine Deaminase isolated and purified from Calf Intestine.
Freund's complete adjuvant is used in the first step of the immunization procedure.
|Specificity||The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
|Formulation||PBS, pH 7.2 without preservatives and foreign proteins.
State: Azide Free
State: Lyophilized IgG fraction.
|Reconstitution Method||Restore by adding 1.0 ml of sterile distilled water.|
|Purification||Ammonium Sulphate Precipitation and Ion Exchange Chromatography.|
|Storage||Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
|Stability||Shelf life: one year from despatch.|
|Background||ADA (Adenosine deaminase) is an enzyme involved in the purine catabolic pathway. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Defects in ADA are a cause of autosomal recessive severe combined immuno-deficiency (SCID).|
|Synonyms||Adenosine aminohydrolase, ADA|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.