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EIF4B pSer422 Rabbit Polyclonal Antibody

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SKU
AP09472PU-N
EIF4B pSer422 rabbit polyclonal antibody, Aff - Purified
Size:
100 ul 50 ul
Conjugation:
Unconjugated
  $430.00
2 Weeks*
Bulk/Customize

View other "EIF4B or NP_001408" antibodies

Specifications
Specifications
Product Data
Application IHC, WB
Recommended Dilution Western Blot: 1/500-1/1000.
Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity Human, Mouse
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Synthetic phosphopeptide derived from Human eIF4B around the phosphorylation site of Serine 422 (T-G-Sp-E-S).
Specificity This antibody detects endogenous levels of eIF4B only when phosphorylated at Serine 422.
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State: Aff - Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation Unconjugated
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Blue Ice
Gene Name eukaryotic translation initiation factor 4B
Database Link
Background eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
Synonyms Eukaryotic translation initiation factor 4B, eIF-4B
Note Molecular Weight: 80 kDa
Reference Data
Protein Categories Intracellular Proteins
Protein Pathways mTOR signaling pathway
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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