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GFP (Ads. to Hu, Ms, Rt Serum Proteins) Rabbit Polyclonal Antibody

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SKU
AP09218BT-N
Anti-GFP (RABBIT) Antibody Biotin Conjugated, 100 ug
Size:
100 ug
Conjugation:
Unconjugated FITC HRP Unconjugated
  $825.00
3 Days*
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Specifications
Specifications
Product Data
Application ELISA, IF, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:5,000
ELISA: 1:10,000 - 1:50,000
Reactivity eGFP, GFP, rGFP
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse and Rat Serum Proteins.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL - lot specific
Purification Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Unconjugated
Storage Store Anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Stability Expiration date is one (1) year from date of receipt.
Shipping Ambient
Database Link
Background GFP Antibody is produced from Green fluorescent protein which is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms rabbit anti-GFP antibody biotin conjugation, biotin conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Note Biotin Conjugated GFP Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Reference Data
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Documents
Resources

file-alt Citations

STRIPAK regulates Slik localization to control mitotic morphogenesis and epithelial integrity
De Jamblinne, Decelle, Dehghani et al
J. Cell Biol. (2020) 219 (11) DOI: 10.1083/jcb.201911035
Cited by 18
AI Summary: In this study, OriGene's anti-GFP antibody (TP401) was used to detect GFP-tagged proteins during immunostaining, aiding in the analysis of protein localization and function related to mitotic morphogenesis and epithelial integrity.
Snippet: … After several washes with PBT, samples were blocked in PBT with 0.1% BSA, followed by incubation overnight at 4°C with primary antibodies against Slik (1:1,000), p-moesin (1:100), GFP (1:100, TP401;OriGene Technologies), cleaved caspase 3 (1:150, no. 9661;Cell Signaling Technology), MMP1 (1:100;1:1 mix of Developmental Studies Hybridoma Bank [DSHB] nos. 3A6B4 and 5H7B11), Ptc (1:1,000, Apa-1;DSHB), Shg (1:200, DCAD2
Controlling Magnesium Corrosion and Degradation-Regulating Mineralization using Matrix GLA Protein
Hong, Zaky, Chong et al
Acta Biomater (2019) 98, 142-151 DOI: 10.1016/j.actbio.2019.05.048
AI Summary: OriGene’s Matrix GLA Protein cDNA plasmid fused with a green fluorescent protein (GFP) tag was used to transfect mammalian cells, enabling the study of MGP’s effects on magnesium corrosion and mineralization in this research.
Snippet: Matrix GLA Protein cDNA plasmid fused with a green fluorescent protein (GFP) tag (OriGene, MD, USA) was transfected both into human embryonic kidney 293 cells (HEK293) via Lipofectamine 2000 (ThermoFisher Scientific, USA), and human umbilical vein endothelial;
Podocalyxin-like 1 Promotes Invadopodia Formation and Metastasis through Activation of Rac1/Cdc42/Cortactin Signaling in Breast Cancer Cells
Lin, Sun, Liao et al
Carcinogenesis (2014)
Cited by 60
AI Summary: OriGene products contributed to this study by providing full-length human PODXL cDNA (PODXL-wt) and full-length human cortactin cDNA (cortactin-wt), which were used to investigate the roles of these proteins in breast cancer cell invasion and metastasis.
Snippet: Full-length human PODXL complementary DNA (PODXL-wt) was obtained from Origene (Rockville, MD, USA) and was subcloned into the pCMV6-DDK vector. ... Full-length human cortactin (cortactin-wt) was obtained from Origene and subcloned into the pCMV6-GFP ... Cite SaveSaving
Showing 1-3 of 3 papers.
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