KLH Rabbit Polyclonal Antibody
|Recommended Dilution||Flow Cytometry: 1/2000 - 1/10000.
Immunofluorescence: 1/500 - 1/2500.
|Immunogen||KLH (Keyhole Limpet Hemocyanin)|
|Specificity||This antibody reacts to Keyhole Limpet Hemocyanin (KLH).|
|Formulation||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer
Label: Tetramethylrhodamine isothiocyanate (TRITC)
Absorption emission: 550 nm / 570 nm
Molar radio: 2.8 moles TRITC per mole of Rabbit IgG
|Reconstitution Method||Restore with 0.1 ml of deionized water (or equivalent).
For extended storage mix product with glycerol to 50%
|Concentration||1,0 mg/ml (by UV absorbance at 280 nm)|
|Storage||Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.|
|Synonyms||Keyhole Limpet Hemocyanine|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.