Horseradish Peroxidase / HRP Sheep Polyclonal Antibody

SKU
AP09103AF-N
Anti-Peroxidase (Horseradish) (SHEEP) Antibody, 2 mL, Serum
  $560.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, IP, WB
Recommended Dilution WB: 1:1,000 - 1:4,000
IHC: User Optimized
ELISA: 1:10,000 - 1:40,000
IP: User Optimized
Reactivity Peroxidase
Antibody Host Sheep
Isotype IgG
Clonality Polyclonal
Immunogen Peroxidase Horseradish
Specificity This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Peroxidase Horseradish.  Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
Concentration 80 mg/mL - lot specific
Conjugation Unconjugated
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Stability Expiration date is one (1) year from date of receipt.
Shipping Ambient
Database Link
Background Peroxidase described as the removal of hydrogen peroxide, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, a response to environmental stresses such as wounding, a and pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Synonyms sheep anti-Peroxidase Antibody, anti-HRP (horseradish) antibody, sheep anti Peroxidase, sheep anti HRP
Note Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Reference Data
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Documents
Resources

file-alt Citations

MicroRNA-21 regulates the viability and apoptosis of diffuse large B-cell lymphoma cells by upregulating B cell lymphoma-2
Liu, Du, Ruan
Exp Ther Med (2017) 14 (5), 4489-4496 DOI: 10.3892/etm.2017.5021
AI Summary: OriGene’s anti-CD38 antibody (cat. no. TA353695) was used as a positive control in immunohistochemistry experiments to validate staining procedures in this study on microRNA-21 and Bcl-2 in diffuse large B-cell lymphoma.
Snippet: Phosphate-buffered saline instead of a primary antibody was considered as a negative control (NC) and a known positive antibody anti-CD38 (1:100;cat. no. TA353695, Origene Technologies, Inc., Rockville, MD, USA) was set as a positive control
IHC WB
Showing 1-1 of 1 papers.
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