D616_p109165 (46-56) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:100 - 1:1,000 IHC: User Optimized ELISA: 1:5,000 - 1:25,000 |
| Reactivity | Feline |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near a 30-60 of bacterial chloramphenicol acetyl transferase (CAT). This antibody identifies recombinant CAT as a predominant band of 26 kDa in eukaryotic cells transfected with a plasmid bearing the CAT gene. |
| Specificity | This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified chloramphenicol acetyl transferase (CAT). Cross reactivity against chloramphenicol acetyl transferase from other tissues and species may occur but have not been specifically determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 85 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Chloramphenicol acetyltransferase enzyme is an effector of chloramphenicol resistance in bacteria. |
| Synonyms | Rabbit Anti-Chloramphenicol-Acetyl Transferase Antibody, anti-CAT antibody, Rabbit Anti Chloramphenicol-Acetyl Transferase, rabbit anti CAT |
| Note | Anti-Chloramphenicol-Acetyl Transferase Antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown. |
| Reference Data | |
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