DYKDDDDK Epitope Tag Rabbit Polyclonal Antibody
$430.00
2 Weeks*
Specifications
| Product Data | |
| Application | WB |
|---|---|
| Recommended Dilution | Western Blot (1/500-1/1000). |
| Reactivity | Mammalian |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | The antiserum was produced by immunizing Rabbit with synthesized peptide containing the Flag epitope (D-Y-K-D-D-D-D-K). |
| Specificity | Flag-Tag Polyclonal Antibody AP08111PU detects Flag-tagged proteins overexpressed in cells. The antibody recognizes the Flag-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells. |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction. |
| Purification | Immunoaffinity Chromatography using epitope-specific peptide. |
| Conjugation | Unconjugated |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Background | Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. |
| Synonyms | FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag |
| Reference Data | |
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Citations
Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma
Luo, Tsai, Chiu et al
Int J Mol Sci (2022) 23 (10) DOI: 10.3390/ijms23105700
Luo, Tsai, Chiu et al
Int J Mol Sci (2022) 23 (10) DOI: 10.3390/ijms23105700
Cited by 11
AI Summary: OriGene contributed to this study by providing the human full-length SREBP1 cDNA, which was constructed into the pEntry mammalian cell expression vector to investigate the role of SREBP1 in leptin-regulated lipid metabolism in nasopharyngeal carcinoma cells.
Snippet: egulates metabolism through an improvement of, and increase in, ATP production, as well as an increase in fatty acid oxidation [14,15,16]. However, the linkage between altered lipid metabolism and tumorigenesis by leptin and the underlying mechanism have not been completely evaluated in NPC. Sterol regulatory element-binding protein 1 (SREBP1) is a key transcriptional factor that regulates the ex...
Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity
Riera-Tur, Schäfer, Hornburg et al
Life Sci Alliance (2022) 5 (3) DOI: 10.26508/lsa.202101185
Riera-Tur, Schäfer, Hornburg et al
Life Sci Alliance (2022) 5 (3) DOI: 10.26508/lsa.202101185
Cited by 28
AI Summary: OriGene's mouse AP-3μ1 expression plasmid (Ref. MR206629) was used in this study to restore AP-3μ1 levels in neurons, enabling the authors to demonstrate that replenishing AP-3μ1 ameliorates lysosomal defects and neurotoxicity caused by amyloid-like aggregating proteins.
Snippet: pmCherry-N1 was used as control. The mouse AP-3μ1 expression plasmid was purchased from OriGene (Ref. MR206629) and consists of AP-3μ1-myc-DDK in pCMV6-ENTRY. For interactome analysis, myc-mCherry, myc-β4-mCherry, myc-β17-mCherry, and myc-β23-mCherry were cloned into pENTR1A-164 between restriction sites NotI and
Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1
Li, Li, Li et al
NPJ Precis Oncol (2021) 5 (1), 20 DOI: 10.1038/s41698-021-00158-3
Li, Li, Li et al
NPJ Precis Oncol (2021) 5 (1), 20 DOI: 10.1038/s41698-021-00158-3
Cited by 22
AI Summary: In this study, OriGene’s anti-GFP (TA150052) and anti-Myc (TA150121) antibodies were used for Western blot analyses to detect tagged proteins, facilitating the investigation of ELF5 acetylation and its role in breast cancer progression.
Snippet: … Hence, the functional role and regulatory mechanism of ELF5 in cancer tumorigenesis and progression are still largely unclear. … Lysine acetylation is a key form of PTMs t;anti-GFP (1:2000 for WB, TA150052, Origene, Rockville, MD, USA), anti-Myc (1:2000 for WB, TA150121, Origene, Rockville, MD, USA),
MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway
Li, Fang, Shen et al
Biochem. Biophys. Res. Commun. (2019) 509 (2), 603-610 DOI: 10.1016/j.bbrc.2018.12.162
Li, Fang, Shen et al
Biochem. Biophys. Res. Commun. (2019) 509 (2), 603-610 DOI: 10.1016/j.bbrc.2018.12.162
AI Summary: OriGene products, specifically anti-Flag and anti-GFP antibodies, were used in this study to detect tagged proteins and monitor gene expression during experiments investigating the role of miR-135a-5p in neuronal differentiation.
Snippet: Primary antibodies used were anti-CD44 (1:200), anti-Flag (1:1000, Origene), anti-CD133 (1:700, Cell Signaling), anti-GFP (1:100, Origene), anti-MAP2 (1:1000, avesLabs) and anti-GFAP (1:100). 2.8. mRNA-sequence analysis
Olfactory receptor 43 reduces hepatic lipid accumulation and adiposity in mice
Wu, Thach, Kim et al
Biochim Biophys Acta Mol Cell Biol Lipids (2019) 1864 (4), 489-499 DOI: 10.1016/j.bbalip.2019.01.004
Wu, Thach, Kim et al
Biochim Biophys Acta Mol Cell Biol Lipids (2019) 1864 (4), 489-499 DOI: 10.1016/j.bbalip.2019.01.004
Targeting the 15-keto-PGE2-PTGR2 axis modulates systemic inflammation and survival in experimental sepsis
Chen, Hee, Liao et al
Free Radic. Biol. Med. (2017) 115, 113-126 DOI: 10.1016/j.freeradbiomed.2017.11.016
Chen, Hee, Liao et al
Free Radic. Biol. Med. (2017) 115, 113-126 DOI: 10.1016/j.freeradbiomed.2017.11.016
Cited by 47
AI Summary: In this study, the FLAG antibody from OriGene was used to detect FLAG-tagged proteins, contributing to the investigation of the molecular mechanisms underlying the role of PTGR2 and 15k-PGE2 in modulating inflammation and survival in experimental sepsis.
Snippet: IκBα, H2AFZ, β-actin, GAPDH and α-tubulin antibodies from Abcam (Cambridge, MA) and Keap1 antibodies from Santa Cruz Biotechnology (Santa Cruz, CA), NRF2 antibody from Cell Signaling Technology (Beverly, MA), and FLAG antibody from OriGene (Rockville
Showing 1-6 of 29 papers.
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