EIF4G1 Rabbit Polyclonal Antibody

SKU AP08089PU-S

EIF4G1 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.; Immunohistochemistry on paraffin sections: 1/50 - 1/100.; Immunofluorescence: 1/100 - 1/200.
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human eIF4G around the phosphorylation site of Serine 1232 (P-V-Sp-P-L).
• Specificity: This antibody detects endogenous levels of total eLF4G protein.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.; State: Aff - Purified; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: eukaryotic translation initiation factor 4 gamma 1
• Database Link:
  Q04637
• Background: eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.
• Synonyms: EIF4G, EIF4GI, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, Eukaryotic translation initiation factor 4 gamma 1, p220

Reference Data