eNOS (NOS3) Rabbit Polyclonal Antibody

CAT#: AP08055PU-S

eNOS (NOS3) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from HUVEC cell, using eNOS Antibody (Lane 1 and 2).

• 

  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using eNOS antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human eNOS around the phosphorylation site of Serine 1177 (T-Q-SP-F-S).
• Specificity: eNOS Antibody AP08055PU detects endogenous levels of total eNOS protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: nitric oxide synthase 3
• Database Link:
  Entrez Gene 4846 Human
  UniProt ID P29474
• Background: eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
• Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS