PP2A-alpha (PPP2CA) Rabbit Polyclonal Antibody

CAT#: AP06572PU-N

PP2A-alpha (PPP2CA) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PP2A-alpha antibody in extracts from A549 cells.

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  Immunohistochemistry analysis of PP2A-alpha antibody in Paraffin-embedded Human lung carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 270-320 of Human PP2A-α.
• Specificity: This antibody detects endogenous levels of PP2A-alpha protein.
  (region surrounding Thr301)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 35 kDa
• Gene Name: protein phosphatase 2 catalytic subunit alpha
• Database Link:
  Entrez Gene 5515 Human
  UniProt ID P67775
• Background: In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
• Synonyms: Protein Phospatase 2A, PP2A-alpha, Replication protein C