PDE4D Rabbit Polyclonal Antibody

CAT#: AP06558PU-N

PDE4D rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PDE4D antibody in extracts from COS7 cells treated with Nocodazole 1 ug/ml 16 h.

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  Immunohistochemistry analysis of PDE4D antibody in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 30-70 Human PDE4D.
• Specificity: Thhis antibody detects endogenous levels of PDE4D protein.
  (region surrounding His47)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 88 kDa
• Gene Name: phosphodiesterase 4D
• Database Link:
  Entrez Gene 5144 Human
  UniProt ID Q08499
• Background: Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4D gene maps to chromosome 5q12. The splice variants include isoforms PDE4D1-6.
• Synonyms: PDE4D, DPDE3, cAMP-specific 3',5'-cyclic phosphodiesterase 4D, EC=3.1.4.17, DPDE3, PDE43