ATF2 Rabbit Polyclonal Antibody

CAT#: AP06022PU-N

ATF2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ATF2 antibody at 1/500 dilution Lane 1:Hela cell lysate Lane 2:Mouse kidney tissue lysate Lane 3:Rat brain tissue lysate

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  Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using ATF2 antibody.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Immunoprecipitation: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human ATF2.
• Specificity: This antibody detects endogenous levels of ATF2 protein (region surrounding Val65/47).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~55 to 75 kDa
• Gene Name: activating transcription factor 2
• Database Link:
  Entrez Gene 1386 Human
  UniProt ID P15336
• Background: The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
• Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2