ATF2 Rabbit Polyclonal Antibody

SKU AP06020PU-N

ATF2 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000. ; Immunoprecipitation: 1/50-1/200. ; Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human ATF2.
• Specificity: This antibody detects endogenous levels of ATF2 protein.; (region surrounding Gly56).
• Buffer: Phosphate buffered saline (PBS), pH~7.2; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% by SDS-PAGE); Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Predicted Protein Size: ~55 to 75 kDa
• Gene Name: activating transcription factor 2
• Database Link:
  P15336
• Background: The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2. In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2.
• Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2

Reference Data