ATF2 Rabbit Polyclonal Antibody

CAT#: AP06019PU-N

ATF2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ATF2 antibody at 1/500 dilution Lane 1:Hela whole cell lysate Lane 2:MCF-7 whole cell lysate Lane 3:sp2/0 whole cell lysate Lane 4:Rat brain tissue lysate

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  Immunohistochemistry (IHC) analyzes of ATF2 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 70-120 of Human ATF2.
• Specificity: This antibody detects endogenous levels of ATF2 protein.
  (region surrounding Lys106)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 55 to 75 kDa
• Gene Name: activating transcription factor 2
• Database Link:
  Entrez Gene 1386 Human
  UniProt ID P15336
• Background: Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q32.
• Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2