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DYKDDDDK Epitope Tag Rabbit Polyclonal Antibody

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SKU
AP05574PU-N
DYKDDDDK Epitope Tag rabbit polyclonal antibody, Purified
Size:
100 ug
Conjugation:
Unconjugated
  $795.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, WB
Recommended Dilution ELISA: 1:9000 - 1:450000.
Western Blot: 1:2000 - 1:10000.
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Specificity This antibody is specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. This antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.
In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
Buffer 0.02M Potassium phosphate with 0.15M Sodium chloride pH7.2 containing 0.09% Sodium Azide (NaN3)
State: Purified
State: Liquid purified IgG
Concentration lot specific
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Synonyms FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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