Hemoglobin Sheep Polyclonal Antibody

CAT#: AP05441SU-L

Hemoglobin sheep polyclonal antibody, Purified

Product Images

Human Hemoglobin detected in a red blood cell lysate using Sheep anti Human Hemoglobin antibody (AP05441SU-L). Other bands detected represent different subunits of hemoglobin.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/100-1/1000.
• Reactivities: Bovine, Human
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Native Hemoglobin from Erythrocytes
• Specificity: This antibody is specific for Human Hemoglobin.
  It shows weak (< 10%) cross-reactivity with Bovine Hemoglobin.
• Formulation: PBS containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Hemoglobin is a 66kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction. Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions.
  Mutations in the genes for the hemoglobin protein result in a group of hereditary diseases in humans termed hemoglobinopathies, and include sickle-cell disease and thalassemias.
• Synonyms: Haemoglobin